Plasmid_Backbone
pBS1C_Cot

Part:BBa_K3653018:Design

Designed by: Shay Bamany, Shany Greenstein, Dor Josef, Amir Betker   Group: iGEM20_Technion-Israel   (2020-10-12)


pBS1C_CotC_Sybody15


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 535
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 535
    Illegal NheI site found at 667
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 535
    Illegal BglII site found at 652
    Illegal BglII site found at 5934
    Illegal XhoI site found at 273
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 535
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 535
    Illegal XbaI site found at 16
    Illegal NgoMIV site found at 2073
    Illegal NgoMIV site found at 4399
    Illegal NgoMIV site found at 6309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

No


Source

The plasmid: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4177231/ The Cot: https://link.springer.com/article/10.1186/1475-2859-13-30

References